Raman spectroscopy-based creatinine measurement in urine samples from a multipatient population

Area of Science:

• Analytical Chemistry
• Biomedical Spectroscopy
• Clinical Diagnostics

Background:

• Spectroscopic urinalysis offers advantages over traditional chemical methods.
• Urine creatinine measurement is crucial for standardizing other urine assays.
• Previous spectroscopic studies have focused on specific urine components.

Purpose of the Study:

• To report the first application of Raman spectroscopy for measuring creatinine in unaltered, multi-patient urine samples.
• To evaluate the accuracy and feasibility of Raman spectroscopy for clinical creatinine quantification.
• To establish a foundation for broader spectroscopic urinalysis applications.

Main Methods:

• Utilized near-infrared (NIR) excitation for Raman spectroscopy.
• Employed a hybrid linear analysis (HLA) calibration method.
• Measured creatinine concentrations in unaltered urine samples from a diverse patient population.

Main Results:

• Achieved a root mean squared error of cross-validation (RMSECV) of 4.9 mg/dL for creatinine measurement.
• The reference chemical method had an error of 1.1 mg/dL.
• Demonstrated that Raman spectroscopy can quantify creatinine at clinical levels despite patient variations.

Conclusions:

• Raman spectroscopy is a viable method for measuring creatinine in routine urine samples.
• The technique shows promise for accurate quantification even with inherent biological variability.
• Creatinine measurement via Raman spectroscopy is a critical first step toward advanced spectroscopic urinalysis.