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Updated: Aug 29, 2026
Murine Model of Intestinal Ischemia-reperfusion Injury
Published on: May 11, 2016
[Primary research of the effect of intestinal ischemia/reperfusion injury on Leptin concentrations]
Ji Lin1, Guang-tao Yan, Xiu-hua Hao
1Research Laboratory of Biochemistry, Basic Medical Institute, General Hospital of People's Liberation Army, Beijing 100853, China.
Objective:
To explore the effect of intestinal ischemia/reperfusion injury on Leptin concentrations in serum and adipose tissue, and to find out the role of Leptin in acute inflammatory responses.
Methods:
An intestinal ischemia-reperfusion injury model of rats was established, and used a highly-sensitive murine Leptin radioimmunoassay to check the change of Leptin concentrations in serum and adipose tissue.
Results:
Serum Leptin level (10.82+/-0.83) microg/L significantly decreased after an injury of 60-minute ischemia and 30-minute reperfusion versus pre-experimental serum levels (16.46+/-3.21) microg/L; Leptin level in serum was higher than that in adipose tissue (4.466+/-2.63) mg/100g, and they both showed a similar changeable trend to increase step by step as reperfusion time extended (P=0.047).
Conclusion:
Leptin may be an inflammatory cytokine and may play a role in inflammatory responses such as intestinal ischemia/reperfusion.
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