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Updated: Aug 29, 2026

Controllable Ion Channel Expression through Inducible Transient Transfection
Published on: February 17, 2017
Construction of vectors for inducible gene expression in Lactobacillus sakei and L plantarum
Elisabeth Sørvig1, Sonja Grönqvist, Kristine Naterstad
1Matforsk, Norwegian Food Research Institute, Osloveien 1, N-1430 As, Norway. elisabeth.sorvig@matforsk.no
Abstract:
We have constructed vectors for inducible expression of genes in Lactobacillus sakei and Lactobacillus plantarum. The key elements of these vectors are a regulatable promoter involved in the production of the bacteriocins sakacin A and sakacin P and the genes encoding the cognate histidine protein kinase and response regulator that are necessary to activate this promoter upon induction by a peptide pheromone. The vectors are built up of cassettes that permit easy exchange of all parts through restriction enzyme digestion and ligation. Using beta-glucuronidase as a reporter enzyme, variants of these vectors were compared with each other, and with a corresponding system based on genes involved in the production of nisin. Several of the new vectors permitted tightly controlled and efficient expression of beta-glucuronidase in both L. sakei and L. plantarum.
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