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Determination of the formal reduction potential of Lumbricus terrestris hemoglobin using thin layer
Scott C Dorman1, John P Harrington, Melinda S Martin
1Department of Chemistry, Birmingham Southern College, Birmingham, AL 35254, USA. sdorman@bsc.edu
Abstract:
The formal reduction potential (Eo') of Lumbricus terrestris hemoglobin was determined using thin layer spectroelectrochemistry as 0.073 (+/-0.005) V vs Ag/AgCl (0.281 V vs SHE, standard hydrogen electrode). Nernst plots of Lumbricus terrestris hemoglobin with tris-bipyridinecobalt(II) as a mediator titrant have similar linear slopes as Nernst plots of horse heart myoglobin with hexaamineruthenium(II) as a mediator titrant.

