Related Experiment Video
Updated: Aug 9, 2026

Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates
Published on: March 31, 2012
Bacterial mimics of eukaryotic GTPase-activating proteins (GAPs)
1Department of Biological Chemistry and the Kuhne Minerva Center, Institute of Life Sciences, The Hebrew University of Jerusalem, 91904, Jerusalem, Israel.
Abstract:
Bacterial GTPase-activating proteins (GAPs) subvert their host's eukaryotic Rho GTPases to their own advantage. Studies of bacterial GAPs extend our understanding of the action of eukaryotic GAPs, provide new tools for studies of cytoskeletal dynamics and offer new targets for anti-bacterial drugs.
Related Concept Videos
GTPases and their Regulation
Large G-proteins, also known...
Rab Proteins
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Rab Cascades
Small GTPases - Ras and Rho
Three regulatory proteins control their activity:
Activation and Inactivation of G Proteins
GPCRs Regulate Adenylyl Cylase Activity
Two...

