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Related Experiment Videos

A state-dependent trigger for electrophysiological recording at predetermined membrane potentials.

John N J Reynolds1, Jeffery R Wickens

  • 1Department of Anatomy and Structural Biology, School of Medical Sciences, and the Neuroscience Research Centre, University of Otago, PO Box 913, Dunedin, New Zealand. john.reynolds@stonebow.otago.ac.nz

Journal of Neuroscience Methods
|December 9, 2003
PubMed
Summary

This study introduces a novel electronic threshold discriminator for in vivo intracellular recording. The device reliably triggers electrophysiological recordings at specific membrane potentials in oscillating neuronal systems.

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Area of Science:

  • Neuroscience
  • Biophysics
  • Electronic Engineering

Background:

  • Intracellular recording in neuronal systems with oscillating membrane potentials presents challenges for precise event triggering.
  • Isolating specific neuronal states or responses from endogenous membrane potential fluctuations is crucial for accurate electrophysiological analysis.

Purpose of the Study:

  • To describe the circuitry and construction of a novel electronic threshold discriminator.
  • To detail its application in in vivo intracellular recording for neuronal systems exhibiting membrane potential oscillations.
  • To enable triggering of electrophysiological recording and stimulation at pre-selectable membrane potentials.

Main Methods:

  • Development of a novel electronic threshold discriminator.
  • Comparison of analogue membrane potential waveform with a user-defined threshold.

Related Experiment Videos

  • Output of a logic signal to flag threshold-crossing events.
  • Application in in vivo intracellular recording from spiny projection neurons of the striatum.

    • Main Results:

    • The discriminator reliably triggers electrophysiological recording at pre-selectable membrane potentials.
    • Successfully used to measure membrane properties and trigger electrical stimulation in discrete membrane potential states.
    • Acts as a functional clamp to isolate evoked responses from endogenous fluctuations.

    Conclusions:

    • The novel electronic threshold discriminator is effective for in vivo intracellular recording in oscillating neuronal systems.
    • The device is constructed from cheap, readily available components and requires minimal electronics experience.
    • It can be adapted to isolate discrete events in any oscillatory system.