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Updated: Aug 14, 2026

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
Published on: June 13, 2014
Modulation of intracellular Cl- homeostasis by lectin-stimulation in Jurkat T lymphocytes
Zhong-Fang Lai1, Yu-Zhen Chen, Katsuhide Nishi
1Department of Cell and Biological Pharmacology, Graduate School of Medical Sciences, Kumamoto University, Honjo 1-1-1, Kumamoto 860-0811, Japan. lai-zf@gpo.kumamoto-u.ac.jp
Abstract:
We investigated changes in intracellular Cl(-) concentration ([Cl(-)](i)) during lectin-induced activation and proliferation in human Jurkat T lymphocytes. [Cl(-)](i) was measured using Cl(-) fluorescence dye (N-(6-methoyquinolyl) acetoxy-acetyl-ester, MQAE) methods. Lectins, phytohemagglutinin and concanavalin A, dose-dependently increased [Cl(-)](i) and triggered intracellular Cl(-) oscillation in human Jurkat T lymphocytes. However, some mitochondria metabolism inhibitors, such as m-chlorocarbonylcyanide phenylhydrazone (CCP) and 2,4-dinitrophenol, increased [Cl(-)](i) without triggering any Cl(-) oscillation. Furthermore, both lectins and metabolism inhibitors-induced elevation in [Cl(-)](i) were blocked by removal of extracellular Cl(-) from perfusion solution or by application of anthracene-9-carboxylate, a blocker of Cl(-) channels. Since an extracellular Cl(-)-free condition and application of 9-AC also inhibited PHA-induced proliferation, we suggested that elevation of [Cl(-)](i) via activation of Cl(-) channels and increase in incidence of Cl(-) oscillation would play an important role in modulation of Jurkat T cell activation and proliferation.
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