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Inverted spin method for removing RBCs from BM buffy coat products.
R Buckhalter1, K Watkins, S G Ericson
1Blood and Marrow Transplantation Program of West Virginia University Hospitals Processing Laboratory, Morgantown, WV 26506, USA.
Cytotherapy
|December 9, 2003
Summary
The inverted spin method effectively removes red blood cells (RBCs) from bone marrow (BM) buffy coat products, preserving vital mononuclear cells for transplantation. This RBC depletion technique is safe and efficient for patients with pre-identified RBC antibodies.
Area of Science:
- Hematology
- Transplantation Medicine
- Blood Banking
Background:
- The inverted spin method is a historical blood banking technique for red blood cell (RBC) removal.
- Investigated feasibility of inverted spin for RBC depletion of bone marrow (BM) buffy coat products.
- Applied in cases with pre-identified recipient RBC antibodies against donor RBC antigens.
Purpose of the Study:
- To evaluate the efficacy and safety of the inverted spin method for RBC depletion in BM buffy coat products.
- To assess the recovery of mononuclear cells and CD34(+) cells after RBC depletion.
- To determine the clinical outcomes, including granulocyte recovery and hemolytic reactions.
Main Methods:
- BM buffy coat products were processed using an inverted spin technique in transfer packs.
- Products were centrifuged, RBCs were suspended, and then slowly removed.
- Nine patients received BM products prepared with this method between April 1998 and February 2001.
Main Results:
- A median of 81.2% of RBCs were removed, with a median mononuclear cell recovery of 94.3%.
- Median residual RBC volume was 15.0 mL; median CD34(+) cell dose was 1.9 x 10(6)/kg.
- Granulocyte recovery occurred at a median of 24 days post-infusion; no acute hemolytic reactions were observed.
Conclusions:
- The inverted spin method offers effective RBC depletion from BM buffy coat products.
- It preserves essential mononuclear and CD34(+) cells crucial for engraftment.
- Advantages include no need for additives and use of readily available laboratory equipment.