Related Experiment Video
Updated: Aug 13, 2026

Non-Invasive Model of Neuropathogenic Escherichia coli Infection in the Neonatal Rat
Published on: October 29, 2014
A newly discovered verotoxin variant, VT2g, produced by bovine verocytotoxigenic Escherichia coli
P H M Leung1, J S M Peiris, W W S Ng
1Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong SAR, People's Republic of China.
Abstract:
A new verotoxin (VT) variant, designated vt2g, was identified from a bovine strain of verocytotoxigenic Escherichia coli (VTEC) serotype O2:H25. When vt2g was aligned with published sequences of vt2 and vt variants, it exhibited the highest DNA sequence homology with vt2 and vt2c. However, vt2g was not detected by vt2-specific primers and probes, although it was partially neutralized by an antiserum to the VT2A subunit. VT2g was cytotoxic for Vero and HeLa cells and was not activated by mouse intestinal mucus. The vt2g gene was detected in 3 of 409 (0.7%) bovine VTEC strains, including serotypes O2:H25, O2:H45 and Ont:H-.
More Related Videos
07:58Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
09:44Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
Related Concept Videos
Viral Replication: Lytic Cycle
Bacterial Toxins
Regulation of Bacterial Virulence
Botulism
Bacterial Gastroenteritis
Cholera