Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Peripheral blood cell separation through surface-modified polyurethane membranes.

Akon Higuchi1, Shin-ichi Yamamiya, Boo Ok Yoon

  • 1Department of Applied Chemistry, Seikei University, 3-1 Kichijoji Kitamachi 3, Musashino, Tokyo 180-8633, Japan. higuchi@ch.seikei.ac.jp

Journal of Biomedical Materials Research. Part A
|December 9, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microvessel Maturation Correlates With Doppler Ultrasound Indices and Vascular Invasion in Follicular Thyroid Tumors.

Anticancer research·2026
Same author

Injectable alginate hydrogels improve the effects of subretinal hiPSC-derived RPE cell therapy on retinal degeneration in rats.

Materials today. Bio·2026
Same author

Cholesterol analogs modulate lipid nanoparticle performance for mRNA delivery after lyophilization and enable ocular disease therapy.

Materials today. Bio·2026
Same author

Correction: Designed peptide-grafted hydrogels for human pluripotent stem cell culture and differentiation.

Journal of materials chemistry. B·2026
Same author

Enrichment of colon cancer stem cells via polymeric porous filters with different zeta potentials.

Regenerative biomaterials·2026
Same author

Association between shift work and periodontal disease in the Japanese manufacturing industry: A propensity score analysis.

Community dental health·2026

Surface-modified polyurethane membranes selectively separate blood cells. Different functional groups on the membranes control the permeation and recovery of specific cells like CD34(+) stem cells.

Area of Science:

  • Biomaterials Science
  • Cell Separation Technology
  • Surface Chemistry

Background:

  • Peripheral blood cell separation is crucial for diagnostics and therapeutics.
  • Polyurethane (PU) membranes offer potential for cell separation applications.
  • Tailoring membrane surface chemistry can influence cell adhesion and permeation.

Purpose of the Study:

  • To investigate the efficacy of surface-modified polyurethane membranes for selective cell separation from peripheral blood.
  • To evaluate the impact of different functional groups on membrane performance for isolating specific blood cell populations.

Main Methods:

  • Peripheral blood cells were passed through unmodified and surface-modified polyurethane membranes (PU-SO(3)H, PU-N(C(2)H(5))(2), PU-NHC(2)H(4)OH, PU-COOH).
  • Cell permeation ratios and adhesion properties were analyzed for red blood cells, platelets, T cells, B cells, and CD34(+) cells.

Related Experiment Videos

  • Membrane surface treatments, including human albumin solution, were employed to optimize cell recovery.

    • Main Results:

    • Unmodified and PU-SO(3)H membranes allowed passage of red blood cells and platelets.
    • PU-N(C(2)H(5))(2) and PU-NHC(2)H(4)OH membranes showed preferential passage of red blood cells.
    • CD34(+) cell adhesiveness was highest, followed by T cells, B cells, platelets, and red blood cells.
    • PU-COOH membranes treated with albumin enabled recovery of CD34(+) cells, while unmodified PU membranes isolated red blood cells and platelets.

    Conclusions:

    • Surface modification of polyurethane membranes significantly alters their cell separation capabilities.
    • The choice of functional groups dictates the selective permeation and adhesion of different blood cell types.
    • These findings highlight the potential of functionalized PU membranes for targeted isolation of specific blood cells, including stem cells.