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[Influence of different temperatures on internalization of epidermal growth factor receptor complexes in Tca-8113
Mo-yi Sun1, Bao-lin Liu, Yue-gui Jing
1Department of Oral and Maxillofacial Surgery, Stomatological College, Fourth Military Medical University, Xi'an 710032, China. moyisun@fmmu.edu.cn
Objective:
To investigate the effect of different temperatures on internalization of epidermal growth factor receptor complexes in human tongue squamous carcinoma cells(Tca-8113).
Methods:
Human epidermal growth factor r (hEGF) was labeled with 125I according to Chloramine-T method. The quantity of surface binding and internalization of 125I-hEGF in Tca-8113 cells at 4 degrees C and at 37 degrees C was compared respectively with radiobinding assay.
Results:
At 37 degrees C, the amount of EGFR complexes internalization exceeded that of surface binding after 10 min. At 4 degrees C, the level of interlization maintained very low.
Conclusion:
The study indicated that the surface binding of 125I-hEGF at 4 degrees C was steadier than that at 37 degrees C. The study on phenomenon of EGFR complexes internalization provide the experimental basis for selecting anti-EGFR monoclonal antibody in clinic.
