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Published on: July 22, 2011
[Arbitrary primed polymerase chain reaction for the genotypic identification of Streptococcus sanguis group]
Yu-cai Shi1, Wei-dong Zhang, Hui Chen
1Department of Dentistry, Yueqing Hospital, Zhejiang 325600, China.
Objective:
To find out an ideal method used in identification of Streptococcus sanguis group (SSG) strains by arbitrary primed polymerase chain reaction (AP-PCR).
Methods:
AP-PCR was used to distinguish SSG strains by designing 25 bp arbitrary primer 5' AAG AGA GGA GCT AGC TCT TCT TGG A 3'.
Results:
There were great differences in the main band of DNA polymorphism among SSG species. The similar band can be obtained from the different DNA extractions in the same species.
Conclusion:
AP-PCR may be useful in the identification and classification of SSG species.
