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[In situ hybridization analysis of USF1 mRNA expression in odontoblasts]
Li-an Wu1, Ling-ying Wen, Fu-sheng Yang
1Department of Pediatric Dentistry, College of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
Objective:
To detect and verify USF1 mRNA expression in odontoblasts.
Methods:
T-USF1 clone prepared previously was used as template and the desired USF1 cDNA segment was amplified by PCR with specific primers. The segment was labeled with digoxin as a probe and in situ hybridization was performed on the mounting of odontoblasts.
Results:
Evidently positive staining was shown in the cytoplasm of odontoblasts.
Conclusion:
We verified the expression of USF1 mRNA in odontoblasts for the first time and provided evidence for further research.
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