Related Experiment Video
Updated: Jun 26, 2026

Light-driven Enzymatic Decarboxylation
Published on: May 22, 2016
Regeneration of cofactors for use in biocatalysis
Huimin Zhao1, Wilfred A van der Donk
1Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 S. Mathews Avenue, Urbana, IL 61801, USA. zhao5@uiuc.edu
Abstract:
Cofactor-dependent enzymes catalyze many synthetically useful reactions. The high cost of cofactors, however, necessitates in situ cofactor regeneration for preparative applications. After two decades of research, several cofactors can now be effectively regenerated using enzyme or whole-cell based methods. Significant advances have been made in this area in the past three years and include the development of novel or improved methods for regenerating ATP, sugar nucleotides and 3-phosphoadenosine-5'-phosphosulphate. These approaches have found novel applications in biocatalysis.
Related Concept Videos
Bioremediation
Cofactors and Coenzymes
Cofactors and Coenzymes
Role of Reduced Coenzymes NADH and FADH₂
Cofactors and Coenzymes
Cofactors can be metallic ions or organic molecules called coenzymes. These types of helper...
Amino Acid Catabolism

