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Biological assay for tissue kallikrein: comparison with the synthetic substrate S2266
S K Campbell1, K G Marshall, J G Millar
1Department of Renal and Endocrine Medicine, University of Southampton, St. Mary's Hospital, Portsmouth, UK.
Agents and Actions. Supplements
|January 1, 1992
Abstract:
A highly sensitive biological assay for tissue kallikrein is described, using human kininogen as substrate; and quantitation, by radioimmunoassay, of generated kinins. Using purified human urinary kallikrein as a reference standard we have correlated the kininogenase activity of kallikrein with amidase activity as measured by cleavage of the synthetic substrate S2266.