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Updated: Aug 29, 2026

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding
Published on: May 1, 2020
[Expression of connective tissue growth factor gene in the hypertrophic scar and keloid tissue]
Jian-yi Liu1, Shi-rong Li, Shu-xing Ji
1Department of Plastic and Aesthetic Surgery, Southwest Hospital of Third Military Medical University, Chongqing, P. R. China 400038.
Objective:
To explore the effect of connective tissue growth factor on the pathogenesis of hypertrophic scar and keloid tissue.
Methods:
The content of hydroxyproline was determined and the expression of connective tissue growth factor gene was detected by the reverse transcription-polymerase chain reaction and image analysis technique in 5 normal skins, 15 hypertrophic scars and 7 keloid tissues.
Results:
The contents of hydroxyproline in the hypertrophic scar (84.10 +/- 1.76) and keloid tissue (92.38 +/- 2.04) were significantly higher than that of normal skin tissue (26.52 +/- 4.10) (P < 0.01). The index of connective tissue growth factor mRNA in the hypertrophic scar (0.78 +/- 0.63) and keloid tissue (0.84 +/- 0.04) were higher than that of normal skin tissue (0.09 +/- 0.25) (P < 0.01).
Conclusion:
Connective tissue growth factor may play an important role in promoting the fibrotic process of hypertrophic scar and keloid tissue.
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