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Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
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Differences in Ibaraki virus RNA segment 3 sequences from three epidemics.

Yukinori Uchinuno1, Takashi Ito, Yoshiyuki Goto

  • 1Fukuoka Chuo Livestock Hygiene Service Center, Hakata, Fukuoka, Japan.

The Journal of Veterinary Medical Science
|December 11, 2003
PubMed
Summary

Genetic analysis of Ibaraki virus (IBAV) strains reveals distinct evolutionary paths. Japanese IBAV strains show closer genetic ties to Australian and Taiwanese isolates than to Canadian strains, particularly after 1997.

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Area of Science:

  • Veterinary Virology
  • Molecular Epidemiology
  • Arbovirology

Background:

  • Ibaraki virus (IBAV) causes epizootic hemorrhagic disease in cattle.
  • Previous Ibaraki disease (IBAD) outbreaks occurred in Japan in 1959-1960, 1987, and 1997-1998.
  • A novel IBAD form with abnormal births was identified after 1997.

Purpose of the Study:

  • To genetically compare IBAV strains from Japanese epidemics with serotype 2 epizootic hemorrhagic disease virus (EHDV) strains.
  • To investigate the genetic variation in RNA segment 3 of IBAV strains.
  • To understand the evolutionary relationships of IBAV strains across different geographical locations and time periods.

Main Methods:

  • Phylogenetic tree analysis of RNA segment 3.
  • Partial nucleotide sequence analysis of RNA segment 3.
  • Comparative genomics of IBAV and EHDV strains.

Main Results:

  • Significant genetic variation was observed in IBAV strains isolated after 1997.
  • Japanese IBAV strains exhibited closer genetic relatedness to Australian and Taiwanese strains.
  • The Canadian EHDV strain was genetically more distant from the Japanese, Australian, and Taiwanese strains.

Conclusions:

  • Phylogenetic analysis of RNA segment 3 is crucial for understanding IBAV evolution.
  • Geographical origin and epidemic period significantly influence IBAV genetic diversity.
  • The findings provide insights into the molecular epidemiology of Ibaraki virus and related orbiviruses.