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Updated: Aug 4, 2026

08:36
In Vitro Assay to Study Tumor-macrophage Interaction
Published on: August 1, 2019
A comparison of the different methods available for determining BCG-macrophage interactions in vitro, including a new
N Fazal1, R Bartlett, D A Lammas
1Department of Immunology, University of Birmingham, UK.
FEMS Microbiology Immunology
|December 1, 1992
Abstract:
Different methods of determining BCG viability based on colony forming unit (CFU) counting and radio-isotope labelling were comparatively assessed. These included radio-isotope labelling with [3H]uracil, [3H]uridine, [3H]glycerol, and CFU counting, by both agar plate dilution, and microcolony counting in broth. The sensitivity ranges of the different techniques were determined in both macrophage-free and macrophage-treated systems and used to assess the anti-mycobacterial potential of human monocyte-derived macrophages following BCG infection.

