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Updated: Aug 29, 2026

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation
Published on: January 28, 2021
Association of cholesteryl ester transfer protein activity and TaqIB polymorphism with lipoprotein variations in
Katsunori Ikewaki1, Hiroshi Mabuchi, Tamio Teramoto
1Department of Cardiology, Jikei University School of Medicine, Tokyo, Japan.
Abstract:
Cholesteryl ester transfer protein (CETP) facilitates the transfer of cholesteryl ester from high-density lipoprotein (HDL) to apolipoprotein (apo)B-containing lipoproteins, whereby it potentially regulates steady-state concentrations of HDL-cholesterol (HDL-C), as well as low-density lipoprotein-cholesterol (LDL-C). We performed a multicenter trial to assess the association of CETP activity with plasma lipoprotein levels in 591 Japanese subjects. Women had significantly higher CETP activity (15%) and mass (24%) compared to men. For both genders CETP activity was negatively correlated with HDL-C and HDL(2)-C, but positively correlated with LDL-C. B2 allele frequency in TaqIB polymorphism was 40%, with no gender difference. TaqIB genotypes were significantly associated with CETP activity and HDL-C level (both P <.001). B1B1 had the highest CETP activity and the lowest HDL-C concentrations, whereas B2B2 had the lowest CETP activity and the highest HDL-C concentrations. However, no statistically significant differences in triglycerides (TG) or LDL-C were observed across TaqIB genotypes. Multivariate analysis revealed that determinants of HDL-C were age, gender, body mass index (BMI), smoking, alcohol intake, exercise, CETP activity, and TG, and for LDL-C were BMI, age, and CETP. These data demonstrate that CETP activity is a significant determinant of HDL-C and LDL-C levels and that TaqIB CETP gene polymorphism affects CETP activity and HDL-C level in Japanese population examined.
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