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Updated: Aug 29, 2026
Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
Published on: June 30, 2019
Distribution and expression of non-muscle myosin light chain kinase in rabbit livers
Hua-Qing Zhu1, Yuan Wang, Ruo-Lei Hu
1Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei 230032, Anhui Provience, China.
Aim:
To study the distribution and expression of non-muscle myosin light chain kinase (nmMLCK) in rabbit livers.
Methods:
Human nmMLCK N-terminal cDNA was amplified by polymerase chain reaction (PCR) and was inserted into pBKcmv to construct expression vectors. The recombinant plasmid was transformed into XL1-blue. Expression protein was induced by IPTG and then purified by SDS-PAGE and electroelution, which was used to prepare the polycolonal antibody to detect the distribution and expression of nmMLCK in rabbit livers with immunofluorescene techniques.
Results:
The polyclonal antibody was prepared, by which nmMLCK expression was detected and distributed mainly in peripheral hepatocytes.
Conclusion:
nmMLCK can express in hepatocytes peripherally, and may play certain roles in the regulation of hepatic functions.
