Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Recombineering and its application].

Jian-Guang Zhou1, Xin Hong, Cui-Fen Huang

  • 1Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100850, China. zhoujgx@public.bta.net.cn

Yi Chuan Xue Bao = Acta Genetica Sinica
|December 13, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

EXOC8 of Epinephelus coioides involved in SGIV infection via innate immunity and apoptosis.

Developmental and comparative immunology·2025
Same author

The regulatory role of Epinephelus Coioides miR-21 in the infection and replication of iridovirus SGIV.

Virology·2024
Same author

Epinephelus coioides Sec3 promotes Singapore grouper iridovirus infection by negatively regulates immune response.

Fish & shellfish immunology·2024
Same author

Transcriptomic analysis revealed the dynamic response mechanism to acute ammonia exposure in the ivory shell, Babylonia areolata.

Fish & shellfish immunology·2023
Same author

YTHDC1 as a tumor progression suppressor through modulating FSP1-dependent ferroptosis suppression in lung cancer.

Cell death and differentiation·2023
Same author

Comparative chloroplast genomics reveals the phylogeny and the adaptive evolution of Begonia in China.

BMC genomics·2023
Same journal

Powers of multiple-testing procedures for identification of genes significantly differentially expressed in microarray experiments.

Yi chuan xue bao = Acta genetica Sinica·2006
Same journal

Analysis of the phylogenetic relationships among several species of Gramineae using ACGM markers.

Yi chuan xue bao = Acta genetica Sinica·2006
Same journal

Differential expression of endogenous ferritin genes and iron homeostasis alteration in transgenic tobacco overexpressing soybean ferritin gene.

Yi chuan xue bao = Acta genetica Sinica·2006
Same journal

Ultrastructure and gene mapping of the albino mutant al12 in rice (Oryza sativa L.).

Yi chuan xue bao = Acta genetica Sinica·2006
Same journal

Inheritance analysis of herbicide-resistant transgenic soybean lines.

Yi chuan xue bao = Acta genetica Sinica·2006
Same journal

Molecular cloning of bovine FABGL gene and its effects on bovine bioeconomic traits.

Yi chuan xue bao = Acta genetica Sinica·2006
See all related articles

Recombineering, a genetic engineering method using phage lambda Red, enables efficient DNA modification in E. coli with short homology. This powerful technique facilitates genomic experiments without RecA or specific restriction sites.

Area of Science:

  • Molecular Biology
  • Genetics
  • Microbiology

Background:

  • Functional genomics requires advanced genetic engineering tools.
  • Traditional methods often involve complex DNA intermediates and specific restriction sites.
  • The bacteriophage lambda Red recombination system offers a novel approach.

Purpose of the Study:

  • To review the bacteriophage lambda Red recombinase system.
  • To discuss the progression and applications of recombineering.
  • To highlight the advantages of recombineering for genetic engineering.

Main Methods:

  • Utilizes homologous recombination mediated by phage-encoded functions.
  • Employs short DNA homologies (< 50 bp).
  • Does not require plasmid or phage DNA intermediates; uses synthetic oligonucleotides or PCR products.

Related Experiment Videos

Main Results:

  • Achieves highly efficient genetic engineering in Escherichia coli.
  • Functions effectively in the absence of RecA.
  • Allows insertion, deletion, cloning, and substitution of genomic DNA sequences at any position.
  • Facilitates complex genomic experiments.

Conclusions:

  • Recombineering is a powerful and versatile genetic engineering system.
  • It offers significant advantages over traditional methods for manipulating bacterial genomes.
  • The lambda Red system provides a highly efficient platform for functional genomics research.