[Polymorphism of the STR system LPL in the Silesian population]
Kornelia Droździok1, Jadwiga Kabiesz
1Katedry Medycyny Sadowej Slaskiej AM w Katowicach.
Abstract:
This paper shows the results of locus LPL in the population of Upper Silesia. The examinations were performed in a group of 147 unrelated adults. The following gene frequencies were observed: LPL*9 = 0.0578; LPL* 10 = 0.4048; LPL* 11 = 0.2517, LPL* 12 = 0.2449 i LPL* 13 = 0.0408. DNA was isolated using Blood DNA Prep Plus of A&A Biotechnology. Amplification was carried out in a Perkin Elmer GeneAmp PCR System 2400 thermal cycler, using the GenePrint STR System LPL (8p22) of Promega Corporation, Madison, WI, USA. PCR products were electrophoretically separated by high-resolution polyacrylamide gel--GDG of Perkin Elmer. Gels were stained by the silver method. Test chi 2, exact and Carmody's were used for statistical estimation. The identification values of the system are: PD, Ht, PM, MEC, PIC, MEP. The analysis of studies has shown that the examined population is in the genetic equilibrium conformable to the Hardy-Weinberg's principle. The estimated gene frequencies are similar to those observed in two Polish populations.
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