Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Engineered adipose tissue supplied by functional microvessels.

Joerg Borges1, Matthias C Mueller, Nestor Torio Padron

  • 1Department of Plastic and Hand Surgery, Medical Center, University of Freiburg, Germany. borges@ch11.ukl.uni-freiburg.de

Tissue Engineering
|December 13, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of a Novel Thiol-Norbornene-Functionalized Gelatin Hydrogel for Bioprinting of Mesenchymal Stem Cells.

International journal of molecular sciences·2022
Same author

The Edmonton Obesity Staging System Predicts Postoperative Complications After Abdominoplasty.

Annals of plastic surgery·2021
Same author

Proportion of patients eligible for statin therapy substantially varies between different cardiovascular disease risk calculators and guidelines used.

International journal of STD & AIDS·2021
Same author

Assessing the differential impact of chronic CMV and treated HIV infection on CD8+ T-cell differentiation in a matched cohort study: is CMV the key?

AIDS research and therapy·2021
Same author

Efficacy and Cost-Benefit Analysis of Magnetic Resonance Imaging in the Follow-Up of Soft Tissue Sarcomas of the Extremities and Trunk.

Journal of oncology·2021
Same author

Characterization of CRISPR/Cas9 RANKL knockout mesenchymal stem cell clones based on single-cell printing technology and Emulsion Coupling assay as a low-cellularity workflow for single-cell cloning.

PloS one·2021
Same journal

Change in u.s. Patent infringement law.

Tissue engineering·2009
Same journal

Functional reconstruction of the jaw bones using poly(l-lactide) mesh and autogenic particulate cancellous bone and marrow.

Tissue engineering·2009
Same journal

Effect of the structure of bone morphogenetic protein carriers on ectopic bone regeneration.

Tissue engineering·2009
Same journal

Cross-linking of gelatin with carbodiimides.

Tissue engineering·2009
Same journal

Early treatment of diabetes with porcine islets in a bioartificial pancreas.

Tissue engineering·2009
Same journal

Permeability of filters used for immunoisolation.

Tissue engineering·2009
See all related articles

Transplanting human endothelial cells with preadipocytes into a chick embryo model successfully created a vascularized adipose tissue implant. This demonstrates a novel method for engineering functional, three-dimensional tissue constructs.

Area of Science:

  • Tissue Engineering
  • Vascular Biology
  • Adipose Tissue Development

Background:

  • Achieving volume-persistent adipose tissue in vivo requires early vascularization post-transplantation.
  • Cotransplanting preadipocytes with endothelial cells shows potential for rapid capillary network formation.

Purpose of the Study:

  • To investigate the in vivo vascularization of adipose tissue constructs.
  • To assess the integration of engineered microvessels with the host circulatory system.

Main Methods:

  • Utilized a chorioallantoic membrane (CAM) model in fertilized White Leghorn eggs.
  • Transferred human dermal microvascular endothelial cell (HDMVEC) spheroids and preadipocytes in a fibrin matrix to the CAM.
  • Explanted and immunohistologically analyzed composites on day 7.

Related Experiment Videos

Main Results:

  • Detected numerous vessels formed by HDMVECs, perfused with chick erythrocytes.
  • Demonstrated continuity of the newly formed capillary system with the host vasculature.
  • Achieved patent connection of engineered microvessels to the host system without exogenous growth factors.

Conclusions:

  • Cotransplantation of endothelial cell spheroids and preadipocytes enables early vascularization of adipose tissue constructs.
  • This method establishes functional microvascular networks integrated with the host circulation.
  • Potential for engineering complex, vascularized three-dimensional adipose tissue implants.