Related Experiment Video
Updated: Aug 29, 2026
Laboratory Estimation of Net Trophic Transfer Efficiencies of PCB Congeners to Lake Trout (Salvelinus namaycush) from Its Prey
Published on: August 29, 2014
Quantitative identification of atrazine and its chlorinated metabolites in plasma
Jill M Brzezicki1, Melvin E Andersen, Brian K Cranmer
1Department of Environmental and Radiological Health Sciences, Colorado State University, Ft Collins, Colorado 80523, USA. jbrz@colostate.edu
Abstract:
The objective of this study was to develop an analytical method to detect and quantitate the chlorotriazine herbicide atrazine (ATRA), and its chlorinated metabolites [desethylatrazine (DE-ATRA), desisopropylatrazine (DI-ATRA), and diaminochlorotriazine (DACT)] in plasma. Control plasma separated from whole rat blood was fortified with known concentrations of ATRA, DE-ATRA, DI-ATRA, and DACT. These compounds were extracted from the plasma using a liquid-liquid extraction technique, and the resulting extracts were derivatized with tetrabutyl ammonium hydroxide and methyl iodide to produce methylated derivatives of ATRA and its chlorinated metabolites. Derivatized samples and standards were analyzed using gas chromatography-mass spectrometry with selected ion monitoring. Recoveries of fortified plasma samples ranged from 84% to 97% and were validated to 100 ng/mL. This analytical method was subsequently verified in a small-scale animal study to determine time course concentrations of chlorotriazines in plasma following a single oral gavage dose of ATRA to female Sprague Dawley rats.
