Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Nucleotide Excision Repair

Nucleotide Excision Repair

The DNA Replication Fork

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...

Long-patch Base Excision Repair

Long-patch Base Excision Repair

Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

Restarting Stalled Replication Forks

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...

The DNA Replication Fork

The DNA Replication Fork

No description available

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An Investigation into the Low Use Rate of Immediate Sequential Bilateral Cataract Surgeries in the U.S.

Journal of cataract and refractive surgery·2026

Same author

Comprehensive Adult Medical Eye Evaluation Preferred Practice Pattern®.

Ophthalmology·2026

Same author

Comparative long-term outcomes of vitrectomy combined with anterior chamber intraocular lens to intra-scleral haptic fixation of posterior chamber intraocular lens.

International journal of retina and vitreous·2024

Same author

Risk factors for complications in resident-performed cataract surgery: A systematic review.

Survey of ophthalmology·2024

Same author

Traumatic cataract induced by improper use of a percussion massage gun.

American journal of ophthalmology case reports·2024

Same author

Metabolic and proteomic indications of diabetes progression in human aqueous humor.

PloS one·2023

Same journal

Light adjustable lens implantation with cataract surgery in eyes with macular pathology.

Journal of cataract and refractive surgery·2026

Same journal

Comment on: Use of intracameral antibiotics prophylaxis in patients with posterior capsule rupture during cataract surgery: systemic review and meta-analysis.

Journal of cataract and refractive surgery·2026

Same journal

Dehydration and Rehydration Behavior of Ultra-High-Fluence Extracorporeal Cross-Linked Corneal Allogenic Intrastromal Ring Segments (ECO-CAIRS).

Journal of cataract and refractive surgery·2026

Same journal

Multimodal Deep Learning for Predicting Postoperative Vault and Selecting ICL Sizes Using AS-OCT and UBM Images.

Journal of cataract and refractive surgery·2026

Same journal

Reply: Evaluating large language models vs residents in cataract and refractive surgery: comparative analysis using the American Academy of Ophthalmology Self-Assessment Program.

Journal of cataract and refractive surgery·2026

Same journal

Comment on: Evaluating large language models vs residents in cataract and refractive surgery: comparative analysis using the American Academy of Ophthalmology Self-Assessment Program.

Journal of cataract and refractive surgery·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 3, 2026

Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation

Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation

Published on: April 30, 2012

Pop-and-chop nucleofractis.

Rahul T Pandit¹, Thomas A Oetting

¹Department of Ophthalmology and Visual Sciences, University of Iowa Hospitals and Clinics, Iowa City, IA 52242, USA.

Journal of Cataract and Refractive Surgery

|December 13, 2003

Summary

This summary is machine-generated.

Pop and chop is a novel phaco chop technique for endocapsular nucleofractis. This method simplifies nucleus removal, especially for surgeons learning phaco chop or when the nucleus prolapses during hydrodissection.

More Related Videos

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Published on: May 24, 2017

Capturing Common Fragile Site Breaks by Native γH2A.X ChIP

Capturing Common Fragile Site Breaks by Native γH2A.X ChIP

Published on: January 24, 2025

Related Experiment Videos

Last Updated: Apr 3, 2026

Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation

Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation

Published on: April 30, 2012

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Published on: May 24, 2017

Capturing Common Fragile Site Breaks by Native γH2A.X ChIP

Capturing Common Fragile Site Breaks by Native γH2A.X ChIP

Published on: January 24, 2025

Area of Science:

Ophthalmology
Surgical Techniques

Background:

Horizontal chopping is a recognized method for endocapsular nucleofractis.
Managing lens nucleus prolapse during hydrodissection can be challenging.

Purpose of the Study:

To introduce and describe 'pop and chop,' a variant of phaco chop.
To highlight the utility of this technique in specific surgical scenarios.

Main Methods:

The 'pop and chop' technique involves inducing partial extracapsular prolapse of the nucleus before the initial chop.
This facilitates an easier first crack and initial segment removal.

Main Results:

This technique simplifies the initial fragmentation of the lens nucleus.
It proves beneficial in cases of spontaneous nucleus prolapse into the anterior chamber after hydrodissection.

Conclusions:

'Pop and chop' offers an effective approach to endocapsular nucleofractis.
It is particularly advantageous for novice surgeons learning phaco chop and for managing challenging nucleus presentations.