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Preparation and study on the solid inclusion complex of sparfloxacin with beta-cyclodextrin
Jian-Bin Chao1, Hong-Bo Tong, Shu-Ping Huang
1The Test and Analysis Center of Shanxi University, TaiYuan 030006, China. cszyxyxm@sxu.edu.cn
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|December 13, 2003
Summary
Sparfloxacin and beta-cyclodextrin (beta-CD) form a 1:1 inclusion complex. This complex formation was confirmed using various analytical methods, providing insights into its structure and properties.
Area of Science:
- Pharmaceutical Chemistry
- Supramolecular Chemistry
Background:
- Sparfloxacin is a fluoroquinolone antibiotic.
- Beta-cyclodextrin (beta-CD) is a cyclic oligosaccharide known for its ability to form inclusion complexes.
Purpose of the Study:
- To synthesize and characterize a solid inclusion complex of sparfloxacin with beta-cyclodextrin.
- To determine the stoichiometry and formation constant of the complex.
- To elucidate the spatial configuration of the inclusion complex.
Main Methods:
- Coprecipitation method for complex synthesis.
- Characterization using 1H-NMR, 13C-NMR, fluorescence spectroscopy, infrared spectroscopy, thermal analysis (DSC), and scanning electron microscopy (SEM).
- Determination of formation constant via fluorescence and 1H-NMR.
- Structural elucidation using 2D NMR techniques.
Main Results:
- A 1:1 inclusion complex of sparfloxacin with beta-cyclodextrin was successfully synthesized and characterized.
- The formation constant of the complex was quantified.
- The spatial configuration of the sparfloxacin-beta-CD complex was proposed based on 2D NMR data.
Conclusions:
- The coprecipitation method effectively yields a stable 1:1 inclusion complex between sparfloxacin and beta-cyclodextrin.
- Spectroscopic and thermal analyses confirm the formation and structure of the complex.
- The study provides a detailed understanding of sparfloxacin-beta-CD supramolecular interactions.