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Updated: Aug 9, 2026
06:48
An Orthotopic Murine Model of Human Prostate Cancer Metastasis
Published on: September 18, 2013
Chromosome 18 suppresses prostate cancer metastases
S S Padalecki1, K S Weldon, X T Reveles
1Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA
Urologic Oncology
|December 13, 2003
Summary
Chromosome 18 suppresses prostate cancer (CaP) growth and metastasis. Introducing chromosome 18 into CaP cells reduced tumor size, bone metastasis, and improved survival in mice.
Area of Science:
- Oncology
- Genetics
- Cancer Biology
Background:
- Prostate cancer (CaP) progression is linked to specific chromosomal losses on 18q.
- Understanding the role of chromosome 18 in CaP is crucial for identifying therapeutic targets.
Purpose of the Study:
- To investigate the tumor-suppressive function of chromosome 18 in prostate cancer.
- To determine if chromosome 18 can inhibit CaP cell growth and metastasis.
Main Methods:
- Microcell-mediated chromosome transfer was used to introduce chromosome 18 into PC-3 prostate cancer cells.
- Hybrid cell lines were evaluated for in vitro growth (anchorage-dependent and independent) and in vivo tumor formation in nude mice.
- Metastasis to bone and extraskeletal sites was assessed after intra-cardiac inoculation in a nude mouse model.
Main Results:
- Hybrid cell lines with intact chromosome 18 showed reduced anchorage-dependent and independent growth in vitro.
- Tumor size was significantly smaller in nude mice injected with hybrid cells compared to control PC-3 cells.
- Introduction of chromosome 18 led to significantly fewer bone metastases and reduced extraskeletal tumor burden, improving mouse survival.
Conclusions:
- Chromosome 18 possesses functional tumor-suppressive properties in prostate cancer.
- Chromosome 18 can inhibit both primary tumor growth and the metastatic potential of CaP cells.
- Identifying the specific genes on chromosome 18 responsible for these effects could lead to novel drug discovery for CaP treatment.
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