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In vitro effects of acellular milk on the bactericidal components of caprine polymorphonuclear neutrophils
Abstract:
The effects of acellular milk on the activity of the microbicidal cationic enzymes of the polymorphonuclear cells of goats were studied in an attempt to explain the phenomenon by which PMN functions fail in mastitis. Assays were undertaken on the myeloperoxidase, lysozyme and elastase activities in a polymorphonuclear cell (PMN) lysate, both in the presence and absence of acellular milk from homologous species. There was a significant decrease (p < 0.05) in the activity of lysozyme, myeloperoxidase and elastase in the presence of acellular milk. Superoxide and H2O2 production following activation of caprine PMNs by lipopolysaccharide (LPS) was significantly reduced (p < 0.05) in the presence of acellular milk. Thus, the microbicidal function of PMNs is significantly impaired in the presence of acellular milk and this may contribute to the development of mastitis in dairy animals.
Insights
Acellular milk significantly impairs the microbicidal function of goat polymorphonuclear cells (PMNs). This reduction in enzyme activity and oxidative burst may contribute to the development of mastitis in dairy animals.
Area of Science:
- Veterinary Immunology
- Animal Biochemistry
Background:
- Mastitis, a significant concern in dairy animals, involves the failure of polymorphonuclear cell (PMN) functions.
- The role of acellular milk in impairing PMN activity remains incompletely understood.
Purpose of the Study:
- To investigate the impact of acellular milk on the microbicidal cationic enzymes within caprine PMNs.
- To elucidate the mechanisms behind PMN functional failure in mastitis.
Main Methods:
- Assays were performed on myeloperoxidase, lysozyme, and elastase activities in PMN lysates.
- Caprine PMNs were activated with lipopolysaccharide (LPS) to measure superoxide and H2O2 production.
- Experiments were conducted in the presence and absence of acellular milk from goats.
Main Results:
- A significant decrease (p < 0.05) in lysozyme, myeloperoxidase, and elastase activity was observed with acellular milk.
- Superoxide and H2O2 production by LPS-activated caprine PMNs was significantly reduced (p < 0.05) in the presence of acellular milk.
- PMN microbicidal function was demonstrably impaired by acellular milk.
Conclusions:
- Acellular milk significantly inhibits the microbicidal capacity of caprine PMNs.
- This impairment of PMN function by acellular milk may be a contributing factor to the pathogenesis of mastitis in dairy animals.
