Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antigen-driven effector CD8 T cell function regulated by T-bet.

Brandon M Sullivan1, Amy Juedes, Susanne J Szabo

  • 1Department of Immunology and Infectious Diseases, Harvard School of Public Health, Harvard Medical School, Boston, MA 02115, USA.

Proceedings of the National Academy of Sciences of the United States of America
|December 16, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibition of salt-inducible kinases reprograms T cells and antitumor immunity in ovarian cancer.

Nature immunology·2026
Same author

Phase I Clinical Trial of Islet Antigen-Specific Plasmid Coexpressing Tolerogenic Proteins Demonstrates Safety in Adults With Type 1 Diabetes.

Diabetes·2026
Same author

Whole tissue spatial cellular analysis reveals increased macrophage infiltration in pancreata of autoantibody positive donors and patients with type 1 diabetes.

bioRxiv : the preprint server for biology·2025
Same author

A Tolerogenic Plasmid Inhibits Autoantibody Production Triggered by Immunization With Human Peptidyl Arginine Deiminase 4.

Arthritis & rheumatology (Hoboken, N.J.)·2025
Same author

Joint analysis of the nPOD-Virus Group data: the association of enterovirus with type 1 diabetes is supported by multiple markers of infection in pancreas tissue.

Diabetologia·2025
Same author

Enterovirus VP1 protein and HLA class I hyperexpression in pancreatic islet cells of organ donors with type 1 diabetes.

Diabetologia·2025
Same journal

The TaMYB55-TaSnRK1α1-TabZIP9 module confers heat stress tolerance in wheat.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Superstatistics approach to turbulent circulation fluctuations.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

A molecular timescale for evolution of cobamide biosynthesis.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Pierre Chambon, a pioneer of molecular biology and gene regulation in eukaryotes.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Granulosa cell glycogen fuels the avascular corpus luteum.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Synthetic essentiality of TRAIL/TNFSF10 in VHL-deficient renal cell carcinoma.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

The transcription factor T-bet is crucial for CD8+ cytotoxic effector cell generation, impacting immune responses against pathogens. This finding expands our understanding of type 1 immunity regulation in T cells.

Area of Science:

  • Immunology
  • Cellular Biology
  • Molecular Biology

Background:

  • Type 1 immunity involves CD4+ T helper and CD8+ cytotoxic T cells.
  • Transcription factors regulating CD8+ T cell differentiation are not well understood.
  • T-bet is known to regulate CD4+ T helper cell differentiation.

Purpose of the Study:

  • To investigate the role of transcription factor T-bet in CD8+ cytotoxic T cell differentiation.
  • To understand T-bet's function in generating effector CD8+ T cells for type 1 immunity.

Main Methods:

  • Utilized OT-I T cell receptor transgenic mice lacking T-bet.
  • Assessed antigen-driven generation of effector CD8+ cells.
  • Evaluated cytotoxicity and cytokine secretion profiles.

Related Experiment Videos

  • Studied response to lymphocytic choriomeningitis virus infection.

    • Main Results:

    • Mice lacking T-bet showed impaired antigen-driven generation of effector CD8+ cells.
    • Cytotoxicity was diminished in T-bet deficient CD8+ cells.
    • Cytokine secretion profiles were significantly altered.
    • T-bet deficient mice exhibited a poor response to viral infection.

    Conclusions:

    • Transcription factor T-bet is essential for the generation of CD8+ cytotoxic effector cells.
    • T-bet plays a key role in establishing type 1 immunity for both CD4+ and CD8+ T cells.
    • T-bet is a critical regulator of cellular immune responses against pathogens.