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Related Experiment Video

Updated: Jul 9, 2026

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration
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The ethanol switch: a tool for tissue-specific gene induction during plant development.

Yves Deveaux1, Alexis Peaucelle, Gethin R Roberts

  • 1Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK.

The Plant Journal : for Cell and Molecular Biology
|December 17, 2003
PubMed
Summary

Researchers developed an ethanol-inducible system for precise spatial and temporal control of gene expression in plant meristems. This method allows targeted gene manipulation to study plant development.

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Area of Science:

  • Plant Molecular Biology
  • Developmental Biology
  • Gene Regulation

Background:

  • Controlled gene expression is crucial for understanding gene function in plant development.
  • Precise spatial and temporal control of gene activity is a significant challenge.

Purpose of the Study:

  • To establish an ethanol-inducible gene expression system for precise control in plant shoot apical and floral meristems.
  • To demonstrate the system's utility for studying gene function during plant development.

Main Methods:

  • Utilized an ethanol-regulated transcription factor (ALCR) restricted to specific domains via promoters.
  • Employed reporter genes under the control of the alcA promoter for inducible gene activation.
  • Applied the system to mis-express developmental genes like SHOOT MERISTEMLESS (STM) and CYCLIN D3;1 (CYCD3;1).

Main Results:

  • Achieved precise spatial control of gene expression in defined sub-domains of meristems.
  • Demonstrated dynamic and precise temporal control of gene expression using ethanol induction pulses.
  • Showcased system flexibility for simultaneous dual-gene expression or expression in separate domains.

Conclusions:

  • The developed ethanol-inducible system offers robust and flexible tools for precise gene expression control in plants.
  • This system facilitates the functional analysis of genes during plant development, including key developmental regulators.