Isolation of auxin efflux carrier cDNA from cucumber

Cucumber seedlings display not only gravitropism but also peg formation in response to gravity. Gravimorphogenesis is mediated by auxin distribution. As first step to reveal the mechanism that regulates auxin distribution by auxin efflux, we isolated five partial cDNAs of auxin efflux carriers by RT-PCR method. In addition, we isolated two full-length cDNAs (CsPIN2, CsPIN3) from a cucumber cDNA library. CsPIN2, AtPIN3, AtPIN4 and AtPIN7 fall within the same clade. CsPIN3, AtPIN1 and CsPIN1 fall within the same clade. CsPIN5, CsPIN6 and AtPIN2 fall within the same clade. Our phylogenetic analysis of PIN in cucumber and Arabidopsis indicates that cucumber may diversify CsPIN protein compared with AtPIN protein.