Notes on theory and experimental conditions behind two-photon excitation microscopy
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Alessandro Esposito1, Federico Federici, Cesare Usai
1Department of Physics, University of Genoa, 16146 Genoa, Italy.
Microscopy Research and Technique
|December 17, 2003
Abstract:
This report deals with the fundamental quantum physics behind two-photon excitation also providing a link to the experimental consequences exploited in microscopy. The optical sectioning effect is demonstrated as well as the distribution of excitation and of fluorescence emission.
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