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Decolorization of azo dyes by Rhodobacter sphaeroides
Zhi-yong Song1, Ji-ti Zhou, Jing Wang
1School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116023, PR China. songzhy232@yahoo.com.cn
Biotechnology Letters
|December 18, 2003
Abstract:
Rhodobacter sphaeroides AS1.1737 decolorized more than 90% of several azo dyes (200 mg dyes l(-1)) in 24 h. The optimal culture conditions were: anaerobic illumination (1990 1x), peptone as carbon source, temperature 35-40 degrees C and pH 7-8. Intracellular crude enzyme from this strain had azoreductase activity, optimized temperature as 45-50 degrees C, and decolorization kinetics which were consistent with a ping-pong mechanism.