Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

PCR01:32

PCR

Overview
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

Overview
Restriction Enzymes01:11

Restriction Enzymes

Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
Proofreading01:31

Proofreading

Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
Errors During Replication are Corrected by the DNA Polymerase Enzyme

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction to "In Vivo Multiplexed Analysis of Aminopeptidase Activities by Hyperpolarized Molecular Probes for Tumor Diagnostic Applications".

Journal of the American Chemical Society·2026
Same author

Tolerance to extracellular acidic pH facilitates tumor plasticity.

Cell reports·2026
Same author

Incidence and clinical characteristics of zolbetuximab-induced nausea and vomiting in CLDN18.2-positive unresectable advanced or recurrent gastric cancer: a retrospective study.

Journal of pharmaceutical health care and sciences·2026
Same author

In Vivo Multiplexed Analysis of Aminopeptidase Activities by Hyperpolarized Molecular Probes for Tumor Diagnostic Applications.

Journal of the American Chemical Society·2026
Same author

First Report of Morphological Anomalies in Haemaphysalis megaspinosa Ticks from Japan.

Acta parasitologica·2026
Same author

Investigation of the physicochemical and functional properties of poly(2-methacryloyloxyethyl phosphorylcholine)-conjugated aptamers.

Biomaterials science·2025

Related Experiment Video

Updated: Jul 10, 2026

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

Amplified nucleic acid sensing using programmed self-cleaving DNAzyme.

Shinsuke Sando1, Toshinori Sasaki, Keiichiro Kanatani

  • 1Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan. ssando@sbchem.kyoto-u.ac.jp

Journal of the American Chemical Society
|December 18, 2003
PubMed
Summary

A novel target-assisted self-cleavage (TASC) probe enables isothermal, enzyme-free DNA/RNA detection. This catalytic reaction amplifies target sequence information and allows single-nucleotide discrimination using fluorescence.

More Related Videos

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
07:16

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes
05:33

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Published on: July 5, 2024

Related Experiment Videos

Last Updated: Jul 10, 2026

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
07:16

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes
05:33

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Published on: July 5, 2024

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Nucleic Acid Chemistry

Background:

  • Current nucleic acid detection methods often require complex protocols, including PCR amplification, and multiple reagents.
  • There is a need for simpler, more sensitive, and specific detection methods for DNA and RNA targets.
  • Catalytic nucleic acid probes offer potential for signal amplification and simplified detection schemes.

Purpose of the Study:

  • To design and characterize a novel probe system for nucleic acid detection based on target-assisted self-cleavage (TASC).
  • To demonstrate the capability of TASC probes for isothermal, enzyme-free amplification of target sequence information.
  • To develop a fluorescence-reporting TASC probe for sensitive discrimination of single-nucleotide differences in target sequences.

Main Methods:

  • Design of a TASC probe incorporating a target-binding site and a DNAzyme domain.
  • Investigation of the TASC reaction mechanism, where the target DNA/RNA acts as a catalyst.
  • Development of a fluorescence-reporting TASC probe utilizing a Förster Resonance Energy Transfer (FRET) pair (fluorescein/dabsyl) across the cleavage site.

Main Results:

  • The TASC probe undergoes efficient self-cleavage upon hybridization with the target DNA/RNA.
  • The self-cleavage reaction is catalytic, with the probe acting as a substrate and the target as a catalyst, leading to product release.
  • The fluorescence-reporting TASC probe enables mix-and-read detection and discrimination of single-nucleotide variations in the target sequence under isothermal, enzyme/reagent-free conditions.

Conclusions:

  • TASC probes represent a novel and efficient platform for nucleic acid detection and sequence information amplification.
  • The TASC system operates under simple, non-PCR, isothermal conditions, eliminating the need for enzymes and reagents.
  • Fluorescence-reporting TASC probes offer a promising approach for sensitive and specific detection of nucleic acid targets, including single-nucleotide polymorphism (SNP) analysis.