Oriented immobilization of Pseudomonas putida putidaredoxin at a gold (111)-buffer interface: a real time scanning tunnelling microscopy study
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Published on: June 28, 2019
R Mukhopadhyay1, K K Lo, L L Wong
1New Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX1 3QT, UK. rupam@iitk.ac.in
Abstract:
A scanning tunnelling microscopy study of adsorption of wild-type Pseudomonas putida putidaredoxin at a gold (111)-buffer interface has been made in real time. Reversible adsorption has been observed reflecting weak interaction of the wild-type protein with a gold (111) electrode. A genetically engineered mutant, C73S-D58C, which contains a surface thiol, has been used for 'immobilization' and 'orientated adsorption' on the gold surface. The implication of such orientated immobilization in development of a bio-electrode surface has been predicted.
