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Updated: Aug 29, 2026

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
Published on: January 3, 2015
Overexpression of the oncogenic kinase Pim-1 leads to genomic instability
Meejeon Roh1, Bernard Gary, Chisu Song
1Department of Pathology, University of Alabama at Birmingham School of Medicine, Birmingham, Alabama 35294-0007, USA.
Abstract:
Aneuploidy and chromosomal aberrations are hallmarks of most human epithelial malignancies. Here we show that overexpression of the oncogenic kinase Pim-1 in human prostate epithelial cells induces genomic instability by subverting the mitotic spindle checkpoint. Cells overexpressing Pim-1 have a defect in the mitotic spindle checkpoint, abnormal mitotic spindles, centrosome amplification, and chromosome missegregation. Polyploidy and aneuploidy ensue due to a delay in completing cytokinesis. These results define a novel role for elevated Pim-1 expression in promoting genomic instability in human prostate tumors.
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