Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Time correction for computing Michaelis--Menten kinetics.

F Rodriguez1, M Prats

  • 1Centre de Recherches de Biochimie et de Génétique Cellulaires, Toulouse, France.

Computer Applications in the Biosciences : CABIOS
|December 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Brain lesions in a long-term kidney transplant recipient: Primary cerebral lymphoma or cerebral toxoplasmosis?

Neurologia (Barcelona, Spain)·2015
Same author

Spatial learning-related changes in metabolic brain activity contribute to the delimitation of the hippocampal pallium in goldfish.

Behavioural brain research·2015
Same author

Immunohistochemical labelling of cytokines in calves infected experimentally with Mycoplasma bovis.

Journal of comparative pathology·2015
Same author

Immunohistochemical labelling of cyclooxygenase-2 in lung lesions of calves infected with Mycoplasma bovis.

Journal of comparative pathology·2015
Same author

Adverse reactions to immunotherapy are associated with different patterns of sensitization to grass allergens.

Allergy·2015
Same author

Prevalence of thyroid autoimmunity in spanish patients with chronic idiopathic urticaria: a case-control study involving 343 subjects.

Journal of the European Academy of Dermatology and Venereology : JEADV·2015
Same journal

DCA: an efficient implementation of the divide-and-conquer approach to simultaneous multiple sequence alignment.

Computer applications in the biosciences : CABIOS·1998
Same journal

Two applications to facilitate the viewing of database search result files on the Macintosh.

Computer applications in the biosciences : CABIOS·1998
Same journal

BioWish: a molecular biology command extension to Tcl/Tk.

Computer applications in the biosciences : CABIOS·1998
Same journal

The Sequence Alerting Server--a new WEB server.

Computer applications in the biosciences : CABIOS·1998
Same journal

A software tool for the analysis of mass spectrometric disulfide mapping experiments.

Computer applications in the biosciences : CABIOS·1998
Same journal

SAMBA: hardware accelerator for biological sequence comparison.

Computer applications in the biosciences : CABIOS·1998
See all related articles

This study introduces a time correction method for the Michaelis equation to improve the accuracy of Michaelis constant estimation. The new approach corrects for discrepancies between the start of measurement and the reaction initiation time.

Area of Science:

  • Biochemistry
  • Enzyme Kinetics
  • Chemical Kinetics

Background:

  • Accurate estimation of enzyme kinetic parameters, such as the Michaelis constant, is crucial for understanding enzyme mechanisms.
  • Traditional methods can suffer from inaccuracies if the start of data acquisition does not precisely align with the reaction's initiation.
  • This misalignment can lead to erroneous estimations of key kinetic parameters.

Purpose of the Study:

  • To present a novel time correction method for the integrated Michaelis equation.
  • To address and cancel the non-correct estimation of the Michaelis constant.
  • To improve the accuracy of kinetic parameter determination in enzyme assays.

Main Methods:

  • Development of a time correction approach for the integrated Michaelis equation.

Related Experiment Videos

  • Application of the time correction to experimental data.
  • Utilizing a classical least squares iterative procedure for parameter estimation.

    • Main Results:

    • The time correction method effectively addresses the issue of non-coincident time zeros.
    • Accurate estimation of the Michaelis constant is achieved by accounting for the true reaction start time.
    • The classical least squares iterative procedure, combined with time correction, provides reliable kinetic parameter values.

    Conclusions:

    • The proposed time correction is essential for accurate Michaelis constant determination.
    • This method enhances the reliability of enzyme kinetic studies.
    • The approach offers a significant improvement for analyzing experimental data in enzyme kinetics.