Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Depth-resolved multiphoton polarization microscopy by third-harmonic generation.

Dan Oron1, Eran Tal, Yaron Silberberg

  • 1Department of Physics of Complex Systems, The Weizmann Institute of Science, Rehovot 76100, Israel. dan.oron@weizmann.ac.il

Optics Letters
|December 19, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Damselflies overcome color saturation barriers of photonic glasses via pigment loading and refractive index modulation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Phonon-driven wavefunction localization enhances room-temperature single-photon purity in large hybrid lead halide perovskite quantum dots.

Nature communications·2026
Same author

Co-created data governance frameworks for youth mental healthcare: Values, principles, and implementation-A scoping review.

Digital health·2025
Same author

Unraveling Size Dependent Bi- and Tri-Exciton Characteristics in CdSe/CdS Core/Shell Quantum Dots via Ensemble Time Gated Heralded Spectroscopy.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Correlations in the Binding Energy of Triexcitons and Biexcitons in Single CdSe/CdS Nanoplatelets Revealed by Heralded Spectroscopy.

The journal of physical chemistry letters·2025
Same author

Interface Engineering in a CdS-Modified PbS Nanosheet-FAPbI<sub>3</sub> Heterostructure Enabling High-Performance Perovskite Solar Cells.

ACS applied materials & interfaces·2025
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

We developed a new microscopy technique using third-harmonic generation to image the optical anisotropy of materials. This method visualizes the internal structure of biogenic crystals in sea urchin spicules with depth resolution.

Area of Science:

  • Optics
  • Materials Science
  • Biophysics

Background:

  • Optical microscopy is crucial for visualizing material structures.
  • Understanding optical anisotropy is key in various scientific fields.
  • Existing methods may lack depth resolution or specificity for certain materials.

Purpose of the Study:

  • To develop a depth-resolved polarization microscopy technique.
  • To utilize third-harmonic generation (THG) for imaging optical anisotropy.
  • To demonstrate the technique on biogenic crystals.

Main Methods:

  • Employing third-harmonic generation (THG) microscopy.
  • Using a tightly focused circularly polarized beam.
  • Measuring the intensity and polarization of the THG signal.

Related Experiment Videos

Main Results:

  • Achieved depth-resolved imaging of optical anisotropy.
  • THG signal is dominated by positively phase-matched generation in birefringent crystals.
  • Demonstrated successful imaging of biogenic crystals in sea urchin larval spicules.

Conclusions:

  • Third-harmonic generation microscopy provides depth-resolved information on optical anisotropy.
  • The technique is sensitive to the local optical properties of anisotropic media.
  • This method offers a novel approach for studying biogenic and other anisotropic materials.