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Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging
Published on: March 15, 2010
The exocyst component Sec5 is required for membrane traffic and polarity in the Drosophila ovary
Mala Murthy1, Thomas L Schwarz
1Division of Neuroscience, Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Abstract:
The directed traffic of membrane proteins to the cell surface is crucial for many developmental events. We describe the role of Sec5, a member of the exocyst complex, in directed membrane traffic in the Drosophila oocyte. During oogenesis, we find that Sec5 localization undergoes dynamic changes, correlating with the sites at which it is required for the traffic of membrane proteins. Germline clones of sec5 possess defects in membrane addition and the posterior positioning of the oocyte. Additionally, the impaired membrane trafficking of Gurken, the secreted ligand for the EGF receptor, and Yolkless, the vitellogenin receptor, results in defects in dorsal patterning and egg size. However, we find the cytoskeleton to be correctly oriented. We conclude that Sec5 is required for directed membrane traffic, and consequently for the establishment of polarity within the developing oocyte.
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