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Development and validation of a robust specific enzyme mediated assay for phenylalanine in serum
R S Campbell1, G M Brearley, H Varsani
1Department of Clinical Biochemistry, London Hospital Medical College, UK.
Clinica Chimica Acta; International Journal of Clinical Chemistry
|September 30, 1992
Abstract:
The specificity of a phenylalanine dehydrogenase, particularly with respect to cross reactivity toward tyrosine, has been shown to be pH dependent, being minimal at high pH. The dehydrogenase step has been coupled to colorimetric detection of NADH using a tetrazolium salt. The assay shows no significant cross reactivity towards a range of amino acids or drugs and correlates well with an established HPLC technique.