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Related Experiment Videos

Flytrap, a database documenting a GFP protein-trap insertion screen in Drosophila melanogaster.

Reed J Kelso1, Michael Buszczak, Ana T Quiñones

  • 1Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USA.

Nucleic Acids Research
|December 19, 2003
PubMed
Summary

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Flytrap is a database detailing transposable element insertions in Drosophila melanogaster, aiding gene discovery through protein localization data. This resource facilitates research by providing access to insertion lines and their associated gene information.

Area of Science:

  • Genetics
  • Molecular Biology
  • Bioinformatics

Background:

  • Transposable elements are mobile genetic sequences.
  • Understanding their insertion sites is crucial for gene function studies.
  • Drosophila melanogaster is a key model organism in genetic research.

Purpose of the Study:

  • To create a comprehensive, web-enabled relational database of transposable element insertions.
  • To facilitate the study of gene function through protein localization.
  • To provide researchers with a searchable resource for Drosophila melanogaster insertion lines.

Main Methods:

  • Utilizing a P-element carrying a green fluorescent protein (GFP) artificial exon for tagging.
  • Employing inverse-PCR and DNA sequencing to determine tagged gene sequences.

Related Experiment Videos

  • Developing a cellular component ontology based on Gene Ontology consortium rules for protein localization data.

    • Main Results:

    • Established Flytrap, a database containing protein localization micrographs and gene sequence data.
    • Integrated data with Flybase for comprehensive gene information.
    • Enabled searching by BDGP numbers and protein localization patterns.

    Conclusions:

    • Flytrap serves as a valuable resource for Drosophila melanogaster research.
    • The database enhances the study of gene function and localization.
    • It streamlines access to transposable element insertion lines for the scientific community.