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Updated: Aug 29, 2026

MISSION esiRNA for RNAi Screening in Mammalian Cells
Published on: May 12, 2010
Nonspecific, concentration-dependent stimulation and repression of mammalian gene expression by small interfering
Stephan P Persengiev1, Xiaochun Zhu, Michael R Green
1Howard Hughes Medical Institute, Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.
Abstract:
RNA interference is an evolutionarily conserved process in which expression of a specific gene is post-transcriptionally inhibited by a small interfering RNA (siRNA), which recognizes a complementary mRNA and induces its degradation. Currently, RNA interference is being used extensively to inhibit expression of specific genes for experimental and therapeutic purposes. For applications in mammalian cells, siRNAs are designed to be
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