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Updated: Aug 29, 2026

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
The RNA binding domain within the nucleoporin Nup153 associates preferentially with single-stranded RNA
Jennifer R Ball1, Christian Dimaano, Katharine S Ullman
1Department of Oncological Sciences, Huntsman Cancer Institute, The University of Utah, Salt Lake City, Utah 84112, USA.
Abstract:
The nuclear pore protein Nup153 is important for the transport of protein and RNA between the nucleus and cytoplasm. Recently, a novel RNA binding domain (RBD) was mapped within the N-terminal region of Nup153; however, the determinants of RNA association were not characterized. Here we have tested a range of RNAs with different general features to better understand targets recognized by this domain. We have found that the RBD associates with single-stranded RNA with little sequence preference. These results provide new information about a novel RNA binding domain and suggest new models to consider for the contribution of Nup153 to nucleocytoplasmic transport.
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