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Heparinase treatment of RNA before quantitative real-time RT-PCR

Mary Lynn Johnson1, Chainarong Navanukraw, Anna T Grazul-Bilska

  • 1North Dakota State University, Department of Animal and Range Sciences, Fargo, ND, USA. mary.lynn.johnson@ndsu.nodak.edu

Biotechniques
|December 20, 2003
PubMed
Abstract

No abstract available in PubMed .

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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