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Quantitative PCR genotyping assay for the Ts65Dn mouse model of Down syndrome
Dong P Liu1, Cecilia Schmidt, Timothy Billings
1Jackson Laboratory, Bar Harbor, ME, USA.
Biotechniques
|December 20, 2003
Abstract:
The Ts65Dn mouse is a segmentally trisomic model for Down syndrome. Until now, Ts65Dn mice have been identified by the laborious methods of either chromosomal analysis of cultured peripheral lymphocytes or fluorescent in situ hybridization (FISH). We report here a quantitative PCR method for genotyping Ts65Dn mice, as well as a phenotypic description for visually preclassifying mice to be genotyped.
