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Updated: Aug 6, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
Salting-in effects offset MgCL(2)-induced refolding of nucleoside diphosphate kinase
Matsujiro Ishibashi1, Tsutomu Arakawa, Masao Tokunaga
1Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
Abstract:
Previously we reported that halobacterial nucleoside diphosphate kinase can be refolded in the presence of concentrated trimethylamine N-oxide (TMAO) as well as NaCl, indicating that enhancement of compact structure formation by TMAO is sufficient for folding. Here we showed that the refolding effect of MgCl(2) is maximal at 1 M and declines to zero at 2 M, indicating that charge shielding effect of MgCl(2) is offset by its salting-in effect.
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