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Development of a monoclonal antibody capable of detecting prolamine in wheat and oats

J R Gil García1, Jennifer Solano, F Brazil-Dos Santos

  • 1Instituto de Biotecnología, Facultad de Ciencias, Campus Fuentenueva, Universidad de Granada, 18071 Granada, Spain.

Hybridoma and Hybridomics
|December 20, 2003
PubMed

A monoclonal antibody (MAb) capable of detecting the gliadin in wheat flour has been developed. By immunoblot, the monoclonal antibody recognized three bands in the membranes after electrophoresis in an SDS PAGE of the alcohol-soluble proteins of wheat, and only one band in the alcohol extract of the oat flour. ELISA technique can be applied using this monoclonal antibody in the detection of gliadin and avenin in samples from wheat or triticale. The characterization of the antibody shows that it is an IgM class. Sensitivity by ELISA to the gliadin was 80 ng/ml.

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