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Related Experiment Videos

Evolutionarily conserved expression pattern and trans-regulating activity of Xenopus p51/p63.

Yoshiya Tomimori1, Iyoko Katoh, Shun-ichi Kurata

  • 1Molecular Pathophysiology, Department of Moleculo-genetic Sciences, Division of Biomedical Laboratory Sciences, Graduate School of Allied Health Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.

Biochemical and Biophysical Research Communications
|December 20, 2003
PubMed
Summary

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Development, growth & differentiation·2023

The p51/p63 gene, crucial for development, shows conserved functions in frogs. Its deltaN isoform is key in larval skin and facial development, similar to mammals.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • The p51/p63 gene, part of the p53 family, is vital for mammalian development, particularly in skin, limbs, and craniofacial structures.
  • p51/p63 exists in transactivator (TA) and N-terminally truncated (deltaN) forms, with varying expression patterns across species.

Purpose of the Study:

  • To investigate the expression and function of p51/p63 in Xenopus laevis (frog) larval and adult tissues.
  • To compare the conserved roles of p51/p63 in development between amphibians and mammals.

Main Methods:

  • Temporal expression analysis of p51/p63 in Xenopus laevis.
  • Immunohistochemical examination of p51/p63-positive cells in larval skin, limb buds, and maxillofacial tissues.
  • Reporter assays to assess the functional activity of Xenopus deltaN-p51/p63 against human TA-p51/p63.

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Main Results:

  • The deltaN form of p51/p63 showed enhanced transcription during Xenopus premetamorphosis (stages 44-48).
  • p51/p63-positive cells increased in larval skin stratification, and high expression was observed in limb bud and maxillofacial epithelia.
  • Xenopus deltaN-A/gamma p51/p63 demonstrated dominant-negative activity against the human TA-A/gamma isotype.

Conclusions:

  • p51/p63 plays a conserved role in amphibian development, particularly in skin and craniofacial morphogenesis.
  • Tissue-specific regulation of p51/p63 and isotype-specific activities are conserved between mammals and Xenopus.
  • The findings highlight conserved molecular mechanisms underlying vertebrate development.