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H2S generated by heart in rat and its effects on cardiac function
Bin Geng1, Jinghui Yang, Yongfen Qi
1Institute of Cardiovascular Research, Peking University First Hospital, PR China.
Biochemical and Biophysical Research Communications
|December 20, 2003
Summary
Hydrogen sulfide (H2S) is produced in heart tissues and acts as a physiological regulator of cardiac function. This effect, mediated by the KATP channel pathway, was demonstrated in vitro and in vivo.
Area of Science:
- Cardiovascular Physiology
- Biochemistry
- Gasotransmitter Signaling
Background:
- Hydrogen sulfide (H2S) is recognized as a gasotransmitter involved in regulating physiological functions in mammalian brain and vessels.
- Endogenous production of H2S from L-cysteine occurs in various tissues.
Purpose of the Study:
- To investigate the endogenous production of H2S in myocardial tissues.
- To determine the physiological role of H2S in cardiac function and its underlying mechanisms.
Main Methods:
- Assessed the expression of cystathionine gamma-lyase mRNA in myocardial tissues.
- Conducted in vitro and in vivo experiments to evaluate the effects of H2S on cardiac function.
- Utilized glibenclamide, a KATP channel blocker, to investigate the involvement of KATP channels.
Main Results:
- Confirmed the expression of H2S-producing enzyme mRNA in the heart.
- Demonstrated that H2S exerts a negative inotropic effect on cardiac function.
- Observed a decrease in central venous pressure following NaHS injection, partly reversible by glibenclamide.
Conclusions:
- H2S is endogenously produced in the heart and functions as a physiological regulator of cardiac function.
- The negative inotropic effect of H2S in the heart is, in part, mediated by the KATP channel pathway.