Related Experiment Video
Updated: Jul 5, 2026

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
Published on: July 22, 2012
A novel method for rapidly diagnosing the causes of diarrhoea
C S J Probert1, P R H Jones, N M Ratcliffe
1Department of Medicine, Bristol Royal Infirmary, Bristol, UK. c.s.j.probert@bristol.ac.uk
Background:
The microbiological diagnosis of infectious diarrhoea may take several days using conventional techniques. In order to determine whether flatus can be used to make a rapid diagnosis, the volatile organic compounds associated with diarrhoea were analysed.
Methods:
Stool samples were collected from 35 patients with infectious diarrhoea and from six healthy controls. Gaseous compounds were extracted from a headspace using solid phase microextraction and analysed using gas chromatography and mass spectroscopy.
Results:
Characteristic patterns of volatile gases were found for the main causes of infectious diarrhoea in hospitals. Furan species without indoles indicated Clostridium difficile, ethyl dodecanoate indicated rotavirus, ammonia without ethyl dodecanoate suggested other enteric viruses, and the absence of hydrocarbons and terpenes indicated Campylobacter infection.
Conclusion:
These results could be the basis of rapid near patient diagnosis of infectious diarrhoea.
Related Concept Videos
Drugs Affecting GI Tract Motility: Antimicrobials as Antidiarrheal Agents
Serum Laboratory Studies, Stool Test, Breath Test
Rapid Identification of Pathogens
Automated Microbial Diagnostics

